Figure 3.

HDX identified the interaction interface and long-distance stabilization of the protein structure. (A) Relative deuteration differences [DR(FOXO4-DBD) – DR(FOXO4-DBD•DBE)] plotted along the FOXO4-DBD sequence and their evolution in time. Highlighted areas show two regions with large differences in deuteration levels. (B) FOXO4-DBD•DBE structure with highlighted regions showing significant differences in deuteration. Please note that the G₇₄–P₈₇ region of the construct was contributed by the recombinant-production vector and, thus, was not part of the wild-type FOXO4 sequence (see also Figures S3–S7 of Supplementary Materials).