Figure 4.

Mass spectra of transplatin-treated FOXO4-DBD•DBE samples, which were obtained under either (A) non-denaturing (i.e., 1% acetic acid in 7.5 mM ammonium acetate) or (B) denaturing (i.e., 1% acetic acid and 50% methanol in 7.5 mM ammonium acetate solution) conditions. Abbreviations: tPt—bifunctional transplatin conjugate (Pt(NH2)₂); tPt-Cl—monofunctional adduct (Pt(NH2)₂Cl); dsDBD—duplex DNA; ssDBD-F—forward oligonucleotide strand; ssDBD-R—reverse oligonucleotide strand. The FOXO4-DBD sample employed in these experiments lacked three amino acids at the C-terminus (see also Figure S1, Figure S2, Figure S8, Figure S9 of Supplementary Materials.