Table 1.
Hiseq 2000 NGS Analysis | Total reads (100 bp) | 38,390,380 |
Reads after trimming (%), >Q30 | 35,908,618 (93.53) | |
Velvet De Novo Assembly | Optimized Velvet hash value (kmer) | 31 |
Total number of contigs | 15,546 | |
Number of contigs (>1 kb) | 1843 | |
Contig N50 (bp) | 48,718 | |
Length of longest contig (bp) | 418,162 | |
Total bases in contigs (bp) | 35,645,506 | |
Total bases in contigs (>1 kb) | 33,499,878 | |
GC content (%) | 49.79 | |
Gene Prediction | Predicted gene | 12,588 |
Average gene length (bp) | 1911 | |
Average protein length (aa) | 510.29 | |
Average exon per gene | 6.52 | |
Average exon size (bp) | 234.67 | |
Average intron size (bp) | 68.03 |