Table 1.
Properties of Amino Acid Residues of Linkers of Canonical Hsp70s.
| Cytosolic Hsp70s * | E.R. Localized Grp78 | Cytosolic Hsp110s | Grp170s | |
|---|---|---|---|---|
| First Section | G384 (non-polar, hydrophobic) | G (non-polar, hydrophobic) | P (non-polar, hydrophobic) | K (polar, positively charged, hydrophilic) |
| D385 (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) | K (polar, positively charged, hydrophilic) | S (polar, uncharged, hydrophilic) | |
| V386 (non-polar, hydrophobic) | G (non-polar, hydrophobic) | V (non-polar, hydrophobic) | E (polar, negatively charged, hydrophilic) | |
| Eukaryotic Insertion | K (polar, positively charged, hydrophilic) | - | - | - |
| S (polar, uncharged, hydrophilic) | E (polar, negatively charged, hydrophilic) | - | - | |
| E (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) | A (non-polar, hydrophobic) | E (polar, negatively charged, hydrophilic) | |
| N (polar, uncharged, hydrophilic) | T (polar, uncharged, hydrophilic) | F (non-polar, hydrophobic) | V (non-polar, hydrophobic) | |
| Hinge | Q387 (polar, uncharged, hydrophilic) | G (non-polar, hydrophobic) | K (polar, positively charged, hydrophilic) | K (polar, positively charged, hydrophilic) |
| Second Section | D388 (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) | E (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) |
| L389 (non-polar, hydrophobic) | L (non-polar, hydrophobic) | F (non-polar, hydrophobic) | F (non-polar, hydrophobic) | |
| L390 (non-polar, hydrophobic) | V (non-polar, hydrophobic) | S (polar, uncharged, hydrophilic) | L (non-polar, hydrophobic) | |
| Hinge | L391 (non-polar, hydrophobic) | L (non-polar, hydrophobic) | V (non-polar, hydrophobic) | V (non-polar, hydrophobic) |
| Third Section | L392 (non-polar, hydrophobic) | L (non-polar, hydrophobic) | T (polar, uncharged, hydrophilic) | L (non-polar, hydrophobic) |
| D393 (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) | D (polar, negatively charged, hydrophilic) | |
| V394 (non-polar, hydrophobic) | V (non-polar, hydrophobic) | G (non-polar, hydrophobic) | V (non-polar, hydrophobic) |
* Numbering based on E. coli DnaK (P0A648). The residues used in this analysis represent the most frequently occurring residues identified from multiple sequence analysis of approximately 450 sequences using JalView (https://www.jalview.org).