Table 2.

Linker residues involved in Hydrogen bond formation upon ATP/ADP binding.

Nucleotide	Linker Residues	Contact Residues [Domain]	H Bond Length/Å
ATP	D388 (O)	K214 (N) [NBD]	3.078
	L390 (N)	K214 (O) [NBD]	2.761
	L390 (O)	F216 (N) [NBD]	2.797
	L392 (N)	F216 (O) [NBD]	2.894
	L392 (O)	V218 (N) [NBD]	2.980
	D393 (O)	I418 (N) [SBD]	2.913
	D393 (O.D)	V394(N) [linker]	2.794
ADP	L392 (O)	T417 (H) [SBD]	1.771
	V394 (N)	D415 (O) [SBD]	2.593

The predicted bond length values were obtained using protein 3-dimensional models generated using Chimera version 1.1. The templates used for generating the Hsp70 models were as follows: full length Hsp70 in ATP bound form (4po2; [61]), or in ADP-bound state (c2khoA [57]), respectively.