Table 4.

The available PAM sequences for cyanobacterial species highlighted in this study for different Cas variants. Only species with full genomes available are shown. The Cas variants indicated are Streptococcus pyogenes Cas9 (SpCas9), Francisella novicida Cas12a (FnCas12a), Acidaminococcus sp. Cas12a (AcCas12a, and variants AsCas12a-RR and ASCas12a-RVR), Lachnospiraceae bacterium Cas12a (LbCas12a) and Deltaproteobacteria bacterium CasX (CasX). The average number of PAM sites (in brackets) per kb of genome are shown (N = A, T, C, G; V = A, C, G; Y = C, T). Genome data was sourced from The European Nucleotide Archive (https://www.ebi.ac.uk/ena) and The National Centre for Biotechnology Information (https://www.ncbi.nlm.nih.gov/genome).

Cyanobacteria	Genome Size (bp)	SpCas9 (NGG)	FnCas12a (TTN)	AsCas12a and LbCas12a (TTTV)	AsCas12a-RR (TYCV)	ASCas12a-RVR (TATV)	CasX (TTCN)
Arthrospira platensis C1	6,089,210	134	168	24	46	21	33
Arthospira plantensis NIES 39	6,788,435	114	171	37	46	22	33
Chroococcidiopsis thermalis PCC 7203	6,315,792	89	175	38	41	17	34
Cyanothece sp. ATCC 51142	4,934,271	118	221	35	40	23	39
Cyanothece sp. PCC 7822	6,091,620	114	210	33	39	21	36
Gleobacter violaceus PCC 7421	4,659,019	170	89	17	42	6	26
Nostoc punctiforme strain ATCC 29133	8,234,322	114	194	43	40	20	35
Nostoc sp. PCC 7120	6,413,771	119	191	27	39	39	34
Synechococcus elongatus PCC 6301	2,696,255	142	113	10	41	7	28
Synechococcus elongatus PCC 7942	2,695,903	141	113	10	41	6	28
Synechococcus elongatus PCC 11801	2,691,022	139	115	10	41	7	29
Synechococcus sp. PCC 7002	3,008,047	153	163	22	48	11	33
Synechococcus sp. PCC 11901	3,081,514	152	163	22	47	11	33
Synechococcus sp. UTEX 2973	2,690,418	142	113	10	41	6	28
Synechococcus sp. WH 8102	2,434,428	173	86	7	49	4	28
Synechocystis sp. PCC 6803	3,569,561	161	174	23	51	12	32
Thermosynechococcus elongatus BP-1	2,593,857	176	126	13	42	10	27