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. 2019 Oct 3;10(10):672. doi: 10.3390/mi10100672

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The DEP-based microarray device and scheme for isolation of cfDNA from blood. Reproduced with permission from [55]. (A) AC electrokinetic microarray device used with an expanded view of the device materials composition. (B) Microarray with whole blood (red) containing fluorescent DNA (green). (C) Application of the AC electric field causing the fluorescent DNA (green) to be concentrated in the DEP high-field regions on the microelectrodes, while the blood cells (red) move into the DEP low-field regions between the microelectrodes. (D) A fluidic wash removes the blood cells from the microarray under the AC field while DNA remains concentrated in the DEP high-field regions.