Skip to main content
. 2019 Sep 26;7(10):397. doi: 10.3390/microorganisms7100397

Figure 3.

Figure 3

Filopodia and lamellipodia of adhesive stages of Ceratonova shasta genotype IIR in rainbow trout. (A) Presporogonic and sporogonic developmental stages showing abundant static filopodia. (B) Radially distributed filopodia on a stage. (C) Sheet-like lamellipodia with filopodia on the lamellipodia border and also all over the body of the stage. (D) 3D radiating pattern of filopodia. (E) F-actin distribution on a 3D radiating pattern of filopodia. (F) Detail of the parasite surface with small crests. (G,H) Parasite stages with sheet-like lamellipodia and small filopodia projecting in the external margin. Notice the sheet-like lamellipodia located on one side, with opposing single and ramified filopodia over the rest of the parasite body. (I) Detail external margin of lamellipodia with small filopodia on the external border. (J–L) F-actin distribution on stages with lamellipodia. (K) Stage showing F-actin rich small crests (arrows) on the surface. (L) Small F-actin rich filopodia (head arrows) on the external margin of the lamellipodia. All stages were collected from ascites. A–C Light microscopy, D, F–I SEM, E, J–L CLSM (Green-F-actin, phalloidin; blue-nucleic acids, DAPI).