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. 2019 Oct 3;8(10):395. doi: 10.3390/plants8100395

Figure 8.

Figure 8

Systemic gene expression analysis in leaves upon Sys foliar treatment. Quantification of transcripts of early (ProSys, AOS) and late genes (Pin I, Pin II) in leaves distal from the treated ones by real time RT-PCR after 90 min, 3 h and 6 h following 100 pM and 100 nM systemin peptide treatment. Relative quantities are calibrated on samples obtained from tomato leaves spotted with PBS1X (Control). For each gene, RQ variations have been analysed by two-way ANOVA. Different letters denote significantly different values (ProSys: p > 0.05; AOS: p < 0.01 6 h pt; Pin I and Pin II: p < 0.05 3 h pt, p < 0.01 6 h pt). Error bars indicate standard error.