Figure 5.

Identification and expression analysis of transgenic poplar. T1–T3: different transgenic poplar lines; WT, the wild type poplar. (A) Identification of poplar transgenic lines; M, 2000 DNA maker; P, positive control with recombined vector as the template. (B) Transgenic lines can root in the rooting medium containing 50 mg/ml kanamycin. WT seeding is on the left, transgenic seedling is on the right. (C) Relative expression level of ERF38 gene in the WT and transgenic lines by reverse transcriptase quantitative polymerase chain reaction. The error bars represent the standard deviation. Asterisks indicate significant differences between transgenic lines and wild type lines (t test, *P < 0.05).