Figure 3.

ACSL1 siRNA knockdown suppresses TNFα induced GM-CSF production. MDA-MB-231 cells were transfected with ACSL1 siRNA (targeting the human ACSL1 gene expression or scramble siRNA; a control siRNA). (A) After 36 h, real-time PCR was performed to measure ACSL1 expression to test knocking down efficiency. (B) ACSL1 deficient cells were then treated with vehicle and TNFα for 24 h. GM-CSF mRNA was determined by real-time PCR. (C) Secreted GM-CSF in culture media was determined by ELISA. All data are expressed as mean ± SEM (n ≥ 3). *** p < 0.001, **** p < 0.0001 versus vehicle.