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. 2019 Sep 30;8(10):390. doi: 10.3390/plants8100390

Figure 1.

Figure 1

Endpoint polymerase chain reaction (PCR) for manA and egfp coding regions. (A) PCR for the manA coding region. (B) PCR for the egfp coding region. C: Citrus sinensis Osb. x Poncirus trifoliata L. Raf. (Carrizo citrange), S: Citrus paradisi Macf. Duncan grapefruit x Poncirus trifoliata L. Raf (Swingle citrumelo), M: Marker is EZ Load 100 bp Molecular Ruler, P: p2820eGFP plasmid. Note that only Carrizo citrange was included as a negative control because the genomic background is similar in the two and they do not have E. coli manA gene.