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. 2019 Sep 30;8(10):390. doi: 10.3390/plants8100390

Figure 4.

Figure 4

The chlorophenol red pH indicator test for phosphomannose isomerase (PMI). Transgenic, wild-type, and solution alone samples were tested with Murashige and Skoog (1962) plus mannose medium and chlorophenol red pH indicator. A red or purple color indicates negative phosphomannose isomerase activity and an orange or yellow color indicates positive PMI activity. (A) Replicated Carrizo samples, Rows C17, C18, C19, C20, C21, C22, C25, C26: Leaves of Citrus sinensis Osb. x Poncirus trifoliata L. Raf. (‘Carrizo’ citrange) transgenics. Row C: Leaves of wild-type ‘Carrizo’, Row CR: Chlorophenol red solution alone. (B) Replicated Swingle samples, Rows S23, S24, S27, S28: Leaves of Citrus paradisi Macf. Duncan grapefruit x Poncirus trifoliata L. Raf (‘Swingle’ citrumelo) transgenics. Row S: Leaves of Wild-type ‘Swingle’, Row CR: Chlorophenol red solution alone, R1–R3: Three replicates.