Table 1.
Biolistic transformation of Citrus sinensis Osb. x Poncirus trifoliata L. Raf. (Carrizo citrange), the number of transgenic shoots produced, micro-grafted, and tested with PCR.
| Experiment | Paired Shots | Number of Explants | Number of Shoots | Number of Positive Shoots | Number of Shoots Survived/Number Micro-grafted | PCR Positive Shoots |
|---|---|---|---|---|---|---|
| 1 | 1 | 153 | 294 | 2 | 0/1 | 0 |
| 1 | 2 | 126 | 237 | 3 | 1/2 | 1 |
| 2 | 3 | 165 | 288 | 4 | 2/2 | 2 |
| 2 | 4 | 153 | 284 | 2 | 1/1 | 1 |
| 3 | 5 | 159 | 270 | 1 | 1/1 | 1 |
| 3 | 6 | 166 | 214 | 1 | 0/1 | 0 |
| 4 | 7 | 154 | 346 | 1 | 1/1 | 1 |
| 4 | 8 | 80 | 140 | 0 | 0/0 | 0 |
| 5 | 9 | 149 | 319 | 3 | 1/1 | 1 |
| 5 | 10 | 88 | 173 | 2 | 1/1 | 1 |
| Total | - | 1393 | 2565 | 19 | 8/11 | 8/8 |
| Mean ± SE | - | 139.3 ± 9.9 | 256.5 ± 20.5 | 1.9 ± 0.4 | 72.7% survival | 100% |