Table 2.
Biolistic transformation of Citrus paradisi Macf. Duncan grapefruit x Poncirus trifoliata L. Raf (Swingle citrumelo) and the number of transgenic shoots produced, micro-grafted and tested with PCR.
| Experiment | Paired Shots | Number of Explants | Number of Shoots | Number of Positive Shoots | Number of Shoots Survived/Number Micro-grafted | PCR Positive Shoots |
|---|---|---|---|---|---|---|
| 1 | 1 | 103 | 121 | 0 | 0/0 | 0 |
| 1 | 2 | 99 | 187 | 2 | 2/2 | 2 |
| 2 | 3 | 97 | 132 | 1 | 1/1 | 1 |
| 2 | 4 | 110 | 164 | 2 | 0/1 | 0 |
| 3 | 5 | 144 | 205 | 3 | 1/2 | 1 |
| Total | - | 553 | 809 | 8 | 4/6 | 4/4 |
| Mean ± SE | - | 110.6 ± 8.6 | 161.8 ± 15.9 | 1.6 ± 0.5 | 66.7% | 100% |