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. 2019 Oct 6;9(10):575. doi: 10.3390/biom9100575

Table A1.

Sequences of primers used in this study.

Gene Primer Sequence X (Annealing Temperature (°C))
GAPDH F: 5′-TCAAGAAGGTGGTGAAGCAGG-3′
R: 5′-AGCGTCAAAGGTGGAGGAGTG-3′
CAT F: 5′-TGGAGCTGGTAACCCAGTAGG-3′
R: 5′-CCTTTGCCTTGGAGTATTTGGTA-3′
57.0
SOD1 F: 5′-GGTGGGCCAAAGGATGAAGAG-3′
R: 5′-CCACAAGCCAAACGACTTCC-3′
57.0
GSH F: 5′-GGGAGCCTCTTGCAGGATAAA-3′
R: 5′-GAATGGGGCATAGCTCACCAC-3′
57.0
GSH-Px F: 5′-CAGTCGGTGTATGCCTTCTCG-3′
R: 5′-GAGGGACGCCACATTCTCG-3′
57.0
p53 F: 5′-CTTTGAGGTGCGTGTTTGTGC-3′
R: 5′-GGTTTCTTCTTTGGCTGGGGA-3′
51.6
CDK4 F: 5′-ATGGCTACCTCTCGATATGAGC-3′
R: 5′-CATTGGGGACTCTCACACTCT-3′
56.6
Cyclin-D1 F: 5′-GCTGCGAAGTGGAAACCATC-3′
R: 5′-CCTCCTTCTGCACACATTTGAA-3′
60.3
p21 F: 5′-TGTCCGTCAGAACCCATGC-3′
R: 5′-AAAGTCGAAGTTCCATCGCTC-3′
54.9
pRb1 F: 5′-CTCTCGTCAGGCTTGAGTTTG-3′
R: 5′GACATCTCATCTAGGTCAACTGC-3′
66.0
C-myc F: 5′-TGGAACGTCAGAGGAGAAACGA-3′
R: 5′-CTTGAACGGACAGGATGTAGGC-3′
50.0
Caspase-3 F: 5′-CATGGAAGCGAATCAATGGACT-3′
R: 5′-CTGTACCAGACCGAGATGTCA-3′
50.7
Caspase-8 F: 5′-ATTTTGAGATCAAGCCCCACG-3′
R: 5′-GGATACAGCAGATGAAGCAGTCC-3′
66.0
Caspase-9 F: 5′-CTCAGACCAGAGATTCGCAAAC-3′
R: 5′-GCATTTCCCCTCAAACTCTCAA-3′
50.0
Caspase-7 F: 5′-CGGTCCTCGTTTGTACCGTC-3′
R: 5′-CGCCCATACCTGTCACTTTATCA-3′
64.4
cox-2 F: 5′-CTGGCGCTCAGCCATACAG-3′
R: 5′-CGCACTTATACTGGTCAAATCCC-3′
65.0
Bcl-2 F: 5′-ATGTGTGTGGAGAGCGTCAACC-3′
R: 5′-CAGAGACAGCCAGGAGAAATCAA-3′
65.0
NF-κB F: 5′-GAAGCACGAATGACAGAGGC-3′
R: 5′-GCTTGGCGGATTAGCTCTTTT-3′
50.9
PCNA F: 5′-CCAGGGCTCCATCCTCAAGAA-3′
R: 5′-GACGTGGGACGAGTCCATGCT-3′
62.0
TGF-β F: 5′-CAGCGTGCCTAAACTTTATCAGC-3′
R: 5′-TCAGGAGGATGTTTCACATGGA-3′
58.2