Table A1.
Gene | Primer Sequence | X (Annealing Temperature (°C)) |
---|---|---|
GAPDH | F: 5′-TCAAGAAGGTGGTGAAGCAGG-3′ R: 5′-AGCGTCAAAGGTGGAGGAGTG-3′ |
|
CAT | F: 5′-TGGAGCTGGTAACCCAGTAGG-3′ R: 5′-CCTTTGCCTTGGAGTATTTGGTA-3′ |
57.0 |
SOD1 | F: 5′-GGTGGGCCAAAGGATGAAGAG-3′ R: 5′-CCACAAGCCAAACGACTTCC-3′ |
57.0 |
GSH | F: 5′-GGGAGCCTCTTGCAGGATAAA-3′ R: 5′-GAATGGGGCATAGCTCACCAC-3′ |
57.0 |
GSH-Px | F: 5′-CAGTCGGTGTATGCCTTCTCG-3′ R: 5′-GAGGGACGCCACATTCTCG-3′ |
57.0 |
p53 | F: 5′-CTTTGAGGTGCGTGTTTGTGC-3′ R: 5′-GGTTTCTTCTTTGGCTGGGGA-3′ |
51.6 |
CDK4 | F: 5′-ATGGCTACCTCTCGATATGAGC-3′ R: 5′-CATTGGGGACTCTCACACTCT-3′ |
56.6 |
Cyclin-D1 | F: 5′-GCTGCGAAGTGGAAACCATC-3′ R: 5′-CCTCCTTCTGCACACATTTGAA-3′ |
60.3 |
p21 | F: 5′-TGTCCGTCAGAACCCATGC-3′ R: 5′-AAAGTCGAAGTTCCATCGCTC-3′ |
54.9 |
pRb1 | F: 5′-CTCTCGTCAGGCTTGAGTTTG-3′ R: 5′GACATCTCATCTAGGTCAACTGC-3′ |
66.0 |
C-myc | F: 5′-TGGAACGTCAGAGGAGAAACGA-3′ R: 5′-CTTGAACGGACAGGATGTAGGC-3′ |
50.0 |
Caspase-3 | F: 5′-CATGGAAGCGAATCAATGGACT-3′ R: 5′-CTGTACCAGACCGAGATGTCA-3′ |
50.7 |
Caspase-8 | F: 5′-ATTTTGAGATCAAGCCCCACG-3′ R: 5′-GGATACAGCAGATGAAGCAGTCC-3′ |
66.0 |
Caspase-9 | F: 5′-CTCAGACCAGAGATTCGCAAAC-3′ R: 5′-GCATTTCCCCTCAAACTCTCAA-3′ |
50.0 |
Caspase-7 | F: 5′-CGGTCCTCGTTTGTACCGTC-3′ R: 5′-CGCCCATACCTGTCACTTTATCA-3′ |
64.4 |
cox-2 | F: 5′-CTGGCGCTCAGCCATACAG-3′ R: 5′-CGCACTTATACTGGTCAAATCCC-3′ |
65.0 |
Bcl-2 | F: 5′-ATGTGTGTGGAGAGCGTCAACC-3′ R: 5′-CAGAGACAGCCAGGAGAAATCAA-3′ |
65.0 |
NF-κB | F: 5′-GAAGCACGAATGACAGAGGC-3′ R: 5′-GCTTGGCGGATTAGCTCTTTT-3′ |
50.9 |
PCNA | F: 5′-CCAGGGCTCCATCCTCAAGAA-3′ R: 5′-GACGTGGGACGAGTCCATGCT-3′ |
62.0 |
TGF-β | F: 5′-CAGCGTGCCTAAACTTTATCAGC-3′ R: 5′-TCAGGAGGATGTTTCACATGGA-3′ |
58.2 |