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. 2019 Sep 29;8(10):387. doi: 10.3390/plants8100387

Figure 2.

Figure 2

Histological analysis of WT and transgenic fruitlets at 5 days before pollination and at 5, 10 and 20 days after pollination (DAP). Toluidine blue–O staining of WT and transgenic fruitlets at (a) 10 DAP and (b) 20 DAP. (c) Changes in pericarp thickness; (d) number of anticlinal cell layers in pericarp; (e) cell size at 10 DAP and (f) 20 DAP; (g) cell size ratio of 20 DAP/10 DAP in endocarp (single innermost cell layer), mesocarp (middle 50% of the pericarp), and exocarp (2 outer cell layers) of tomato ovaries; (h) number of cells in each category of cell area within each genotype. Flowers were tagged and ovaries from flowers at 5 days before pollination and at 5, 10, and 20 DAP were fixed in 100% methanol, vertically sectioned and stained with 0.04% toluidine blue–O. Digital images of pericarp sections were acquired using AperioScan and analyzed using ImageScope 11. Average cell size (e,f) was calculated by dividing the total number of cells with the area of endocarp, mesocarp, or exocarp. Shown are average ± standard error (n ≥ 3 biological replicates). Different letters above the standard error bars indicate significant difference (at 95% confidence interval) among genotypes within the pericarp section.