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. 2019 Sep 20;55(10):616. doi: 10.3390/medicina55100616

Table 2.

Sequence of primers for RT-PCR.

Gene Product Sequence of Primers Product Length (bp) Annealing Temperature (°C)
Nurr1 5′ CGC CTT TCA CTC TTC TCC TTT A-3´ 481 59
5´ CTT CTT TAA CCA TCC CAA CAG C- 3´
Pitx3 5´ GAG CAC AGT GAC TCG GAG AAG-3´ 462 58
5´ CGG TGA GAA TAC AGG TTG TGA A-3´
α7 nAChRs 5´ AAA ATG CCT AAG TGG ACC AGA A-3´ 401 58
5´ TCA CTG CAG ATC ACC TCA CTC T-3´
β-actin (endogenous control) 5´ ATT TGG CAC CAC ACT TTC TAC A-3´ 379 60
5´ TCA CGC ACG ATT TCC CTC TCA G-3´