Table 2.
Gene Product | Sequence of Primers | Product Length (bp) | Annealing Temperature (°C) |
---|---|---|---|
Nurr1 | 5′ CGC CTT TCA CTC TTC TCC TTT A-3´ | 481 | 59 |
5´ CTT CTT TAA CCA TCC CAA CAG C- 3´ | |||
Pitx3 | 5´ GAG CAC AGT GAC TCG GAG AAG-3´ | 462 | 58 |
5´ CGG TGA GAA TAC AGG TTG TGA A-3´ | |||
α7 nAChRs | 5´ AAA ATG CCT AAG TGG ACC AGA A-3´ | 401 | 58 |
5´ TCA CTG CAG ATC ACC TCA CTC T-3´ | |||
β-actin (endogenous control) | 5´ ATT TGG CAC CAC ACT TTC TAC A-3´ | 379 | 60 |
5´ TCA CGC ACG ATT TCC CTC TCA G-3´ |