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. 2019 Oct 14;7(10):452. doi: 10.3390/microorganisms7100452

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of native INS-15 protein in C. parvum sporozoites. (a) Western blot analysis of native INS-15 protein in crude sporozoite extract in the presence of protease inhibitors using antibodies against the INS-15 domain I (left panel) and pre-immune serum (right panel). Lane M: molecular weight markers, Lane 1: native proteins from sporozoites, Lane 2: purified full-length recombinant INS-15 protein, Lane 3: purified INS-15 domain I. (b) Western blots analysis of native INS-15 protein using anti-INS-15 polypeptide antibodies (left panel) and pre-immune serum (right panel). Lane M: molecular weight markers, Lane 1: native proteins from sporozoites, Lane 2: purified full-length recombinant INS-15 protein.