Figure 5.

Partial neutralization of C. parvum invasion by antibodies against INS-15. Hypochlorite-treated oocysts were pre-incubated with 1:1000, 1:500, 1:200, and 1:100 dilutions of antibodies against the INS-15 domain I (a), the INS-15-specific polypeptide antibodies (b), with pre-immune serum or culture medium alone as controls. Parasite loads per 200× microscope field were calculated and compared among treatment groups. Data presented are mean ± SD from three replicate assays. The * or ** symbol above the treatment group indicates the difference between the treated and untreated groups is statistically significant at p < 0.05 and p < 0.01, respectively.