Figure 3.

Expression of hypA in Aeromonas species determined by RT-qPCR. (A) Relative expression of hypA during phagocytosis by macrophages and under acidic and alkaline culture conditions. Transcript levels of hypA were normalized to the expression of 16S rRNA. (B) Expression fold change of hypA on bacteria grown on acid media respect to the cultured in alkaline media and (C) expression fold change on bacteria phagocytized by macrophages after 4 h of infection respect to control condition on culture alkaline media, calculated using the comparative ΔΔCt method. Error bars indicate standard deviations calculated from three independent experiments. * p-value < 0.05.