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. 2019 Oct 6;10(23):5770–5784. doi: 10.7150/jca.29838

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B7-H3 silencing suppressed OSCC cell proliferation, migration and invasion. A B7-H3 mRNA was evaluated by qRT-PCR 48 h after siRNA transfection. siB7-H3 1 transfection significantly decreased B7-H3 expression at the mRNA level in SCC25 and Cal27 cells. B B7-H3 protein was evaluated by western blot 72 h after siRNA treatment. SiB7-H3 1 transfection significantly decreased B7-H3 expression at the protein level in SCC25 and Cal27 cells. C The proliferation rate was observed by CCK8 assays at 2 days and 5 days after B7-H3 silencing. B7-H3 silencing remarkably slowed the proliferation rate. D Colony formation assays showed that B7-H3 silencing inhibited the colony formation of SCC25 and Cal27 cells. E The migratory ability was evaluated by transwell assays. Silencing B7-H3 reduced the migration of SCC25 and Cal27 cells. F Invasion was evaluated with Matrigel-coated transwell assays. Silencing B7-H3 reduced the invasive ability of SCC25 and Cal27 cells.