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. 2019 Oct 6;10(23):5812–5819. doi: 10.7150/jca.30720

Figure 1.

Figure 1

ERRα regulates OTUB1. HCT116 or Caco2 were transfected with an empty vector or a plasmid overexpressing ERRα, treated with DMSO or XCT790 (10 μM), or transfected with siNC or ERRα siRNA for 48 h. OTUB1 mRNA levels in HCT116 and Caco2 were measured by q-PCR (A, C, E); ERRα and OTUB1 protein levels in HCT116 and Caco2 cells were measured by western blot (B, D, F). Data were normalized to β-actin. The data represent the mean of three independent experiments, and the error bars represent the SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.