. 2019 Oct 25;4(19):18456–18464. doi: 10.1021/acsomega.9b02815

Table 2. Conjugation Condition for Oxidized Dextran and PSPI with Each Conjugate Having the Corresponding Apparent K_i Value.

alternative	PSPI dextran mass ratio	incubation (h)	amount NaBH₃CN	NaBH₄	HCl to neutralize	K_i (μM)	efficiency
free PSPI						0.102 ± 0.025	1.00^a
1	0.59:1	2	100 mM, present throughout	no (stopped by Tris, pH 9.0)		0.277 ± 0.032	0.37
2	0.18:1	22	1 mM, last 2 h	100 mM, 30 min	yes	0.233 ± 0.034	0.44
3	0.18:1	23	no	100 mM, 30 min	yes	0.191 ± 0.025	0.53
4	0.09:1	23	no	100 mM, 30 min	yes	0.171 ± 0.036	0.60
5	0.33:1	23	no	100 mM, 30 min	yes	0.252 ± 0.026	0.41

Reference value.

Table 2. Conjugation Condition for Oxidized Dextran and PSPI with Each Conjugate Having the Corresponding Apparent Ki Value.