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. 2019 Oct 24;4(19):18478–18486. doi: 10.1021/acsomega.9b03208

Figure 4.

Figure 4

Expression levels of autophagy-related proteins and the ratios of autophagic markers (p62 and LC3-II) to GPADH with immunoblotting analysis in IPEC-J2 cells treated with 3-MA and DON. IPEC-J2 cells were cultured with or without DON (1 μM) and 3-MA (5 mM) for 12 h. (A) p62/SQSTM1 and LC3 detected by Western blot in IPEC-J2 cells. (B) Ratio of LC3-II to GAPDH measured by densitometric analysis. (C) Ratio of p62 to GAPDH measured by densitometric analysis. ## means P ≤ 0.01 vs the healthy control group; ** means P ≤ 0.01 vs DON group.