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Effect of 3-MA on DON-induced apoptosis. IPEC-J2 cells were cultured with or without DON (1 μM) and 3-MA (5 mM) for 12 h. (A) Detection of apoptotic and dead cells by fluorescent microscope (4× magnification); (B) GAPDH and Bax detected by Western blot in IPEC-J2 cells. (C) Fluorescence intensity of Annexin V-FITC/PI double-stained IPEC-J2 cells; (D) the ratio of Bax to GAPDH measured by densitometry analysis. ## means P ≤ 0.01 vs control group; ** means P ≤ 0.01 vs DON group.
