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. 2019 Oct 24;4(19):18478–18486. doi: 10.1021/acsomega.9b03208

Figure 7.

Figure 7

Effects of PI3K pathway inhibitor LY294002 and activator 740Y-P on DON-induced apoptosis. IPEC-J2 cells were cultured with or without DON (1 μM) and PI3K pathway activator 740Y-P (20 μM)/inhibitor LY294002 (10 μM) for 12 h. (A) Detection of apoptotic and dead cells by a fluorescent microscope (4× magnification). (B) Fluorescence intensity of Annexin V-FITC/PI double-stained IPEC-J2 cells. ## means P ≤ 0.01 vs control group; ** means P ≤ 0.01 vs DON group.