Table 1.

Spectral properties of the probes in water, pK_a values in the presence and absence of 100mM sugars and dissociation constants of the probes in pH 7.5 phosphate buffer with both glucose and fructose

	o-BMOQBA	m-BMOQBA	p-BMOQBA	BMOQ
λabs (max)/nm	318, 346	318, 347	318, 346	318, 347
λem (max)/nm	450	450	451	453
ϕf	0.46	0.51	0.49	0.54
τf/nsa	26.7	25.9	24.9	27.3
pKa (buffer)	7.90	7.70	7.90	—
pKa (buffer + glucose)	6.62	6.90	6.90	—
pKa (buffer + fructose)	4.80	5.00	5.45	—
KD/mM (glucose)	49.5	1000	430	—
KD/mM (fructose)	0.65	1.8	9.1	—

^aMonoexponential decay time.