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. 2019 Nov 7;12:9435–9447. doi: 10.2147/OTT.S222881

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© 2019 Yang et al.

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GOF-MT SHP2 promotes the proliferation of glioblastoma cells. (A and B) The viability of normal, vector, WT-SHP2 and MT-SHP2 A172 and U87 cells was determined using the MTT assay. (C and D) The cell cycle distribution was detected using flow cytometry. (E and F) A colony formation assay was performed to test the density-independent proliferation of cells in the different groups. Colonies containing >50 cells were counted under a microscope. (G and H) Cell proliferation in agar was used to test the ability of anchorage-independent development. Visible cell colonies (size: >1 mm) were imaged and counted at ×100 magnification. Typical images are shown. The data are presented as the means ± SEM (*P<0.05, **P<0.01, and ***P<0.001).

Abbreviations: GOF, gain-of-function; SHP2, Src homology-2 domain-containing protein-tyrosine phosphatase-2; WT, wild type; MT, mutant; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide.