Figure 5.

GOF-MT SHP2 promotes glioblastoma tumor growth; immunohistochemical staining of tumor specimens. (A and B) Normal, control, WT-SHP2, and MT-SHP2 U87 cells were hypodermically injected into nude mice (n=6 per group). At 4 weeks post injection, the mice were sacrificed, and solid tumors were dissected and imaged. (C and D) After the injection, the tumor size and weight were measured twice per week. (E) H&E staining and IHC assay for SHP2, Ki-67, p-ERK, and p-CREB in tumor tissues obtained from normal, control, WT-SHP2, and MT-SHP2 mice. Images were captured from random fields of view at a magnification of ×400, Scale bar: 50 µm; representative images are shown. The data are presented as the means ± SEM (***P<0.001).

Abbreviations: GOF, gain-of-function; SHP2, Src homology-2 domain-containing protein-tyrosine phosphatase-2; WT, wild type; MT, mutant, IHC, immunohistochemistry; H&E, hematoxylin and eosin; CREB, cAMP-response element-binding protein; ERK, extracellular signal-regulated kinase.