Fig. 1. Construction of yeast integrative plasmid containing human rDNA plus the promoter. a) General process of cutting out human rDNA plus the promoter as an insert and linearizing the template plasmid of the yeast integrative plasmid YIPlac211-TG1 that contains the same sticky ends as the insert as well as connecting these two parts into a new intact plasmid of YIPlac211-TG1-HmrDNA. b) Agarose gel electrophoresis of the linearized YIPlac211-TG1 plasmid as a template and human rDNA with the promoter as an insert. Band 1 in the pink box is human rDNA plus the promoter that was cut out from the pHrP2 plasmid. This insert sequence had the expected size of ∼818 base pairs. Band 2 and band 3 in the green boxes are the linearized YIPlac211-TG1 plasmids with the expected size of ∼3745 base pairs. c) Comparison between wild type and linearized reconstructive plasmid built after inserting human rDNA with the promoter into the yeast integrative plasmid of YIPlac211-TG1.