Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 1;13:486. doi: 10.3389/fncel.2019.00486

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Colnaghi, Russo, Natale, Restelli, Cagnotto, Salmona, Chiesa and Fioriti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) Confocal microscopy of primary neurons. Cells were immunostained for SUMO2/3 (custom antibody, green), synaptophysin (red) and Ubc9 (Abcam #ab21193, magenta). DAPI was used to stain the nuclei. Scale bar, 50 μm. Images were obtained using a 40× objective and displayed as z projection. (B) SIM microscopy. Colored arrowheads indicate the position of the inset shown in panel (C). (C) Merge images represent single stack of SUMO2/3 (green), synaptophysin (red) and Ubc9 (cyan). Scale bar, 0.5 μm. (D) Intensity profile normalized for each channel to 100 (arbitrary unit) using the same color code of SIM merge images and representing the values indicated by the cyan arrow. (E) Confocal microscopy of primary neurons. Cells were immunostained for SUMO2/3 (custom antibody, green), PSD95 (red) and Ubc9 (Abcam #ab21193, magenta). DAPI was used to stain the cell nuclei. Scale bar, 50 μm. Images were obtained using a 40× objective and displayed as z projection. (F) SIM microscopy, colored arrows indicate the position of the inset shown in panel (G). (G) Merge images represent single stack of SUMO2/3 (green), PSD95 (red), and Ubc9 (cyan). Scale bar, 0.5 μm. (H) Intensity profile normalized for each channel to 100 (arbitrary unit) using the same color code of SIM merge images and representing the values indicated by the cyan arrow.