Figure 2.

Post-ETBF myeloid cell subset kinetics and function.

Panel a. CD11b⁺ myeloid cell subset kinetics in the colon. LP CD11b⁺CX3CR1⁺ and CD11b⁺CX3CR1⁻ myeloid cell numbers were quantified in post-ETBF mice. Error bars = SEM, n = 3-6/time point. Days 2–6 were significantly different than day 0 (p < .05). Panel b. In vitro co-culture assay. Sorted CD11b⁺ cell subsets were incubated with CD4⁺ T-cells as described in the Methods. Control T-cells were isolated from naïve APC^min/+ mice whereas experimental T-cells (Bf) were purified from ETBF-inoculated mice. Representative FACS panels are shown for control (T-cells only, top row) and experimental (T-cells + myeloid cells, bottom row). CD4⁺ T-cells were gated on and analyzed for Foxp3 vs IL-17 production. Quantitative data are shown for percent Foxp3⁺ and IL-17⁺ cells for each well. Error bars = SEM, n = 3 mice per group (** and *** denote p < .01 and < 0.001, respectively). Data from one of two repeats with similar results are shown.