Figure 2.
Deletion of grx did not affect bacterial in vitro growth and motility, but slightly decreased the bacterial colony size.
(a-b) In vitro growth and colony size assay of L. monocytogenes wild-type EGD-e, and gene deletion and complementation mutants (Δgrx and CΔgrx). Overnight-grown bacteria were washed and diluted (1:100) in fresh BHI broth (a) or BHI agar medium (b), and incubated at 37°C for 12 hours. Kinetic growth at OD600 nm (a) was measured at 1-h intervals, and bacterial CFU numbers (b) counted at 4-h intervals. Data are expressed as means ± SDs. (c) The bacterial colony morphology grown on the BHI agar plates for 12 hours were observed by using a stereo-microscope. The size of the single bacterial colony was measured of 100 bacteria and data are expressed means ± SDs. **P < 0.01. (d) Bacterial flagellar formation observed by transmission electron microscopy (TEM) were performed on soft agar (0.25%) at 30°C.