Figure 3.

Loss of SDH5 triggers PD-L1 expression by activating ZEB1.

(a–b), ZEB1 overexpression reverses the SDH5-mediated PD-L1 decrease. Increasing amounts of ZEB1 and SDH5 cDNA were cotransfected into H460 (a) or A549 (b) cells for 24 h. Total cell lysates were probed with antibodies against ZEB1 and PD-L1. (c–d), ZEB1 and SDH5 siRNAs were transfected or cotransfected into HCC827 and H1975 cells. PD-L1, ZEB1 and SDH5 proteins were measured by Western blot analysis. (e-f). Flow cytometry (FCM) assay showing the same trend as that of western blot analysis in HCC827 (e) and H1975 (f) cells.