Figure 2.

Beneficial modulation of macrophage population in the TME by lefitolimod. Experiments were performed as described in Figure 1. (a), examples from three representative mice each treated with vehicle or lefitolimod of F4/80⁺ macrophage IHC staining (FFPE sections), display windows are the same as in Figure 1(d). (b), determination of F4/80⁺ macrophages in the tumor border via IHC scoring (*p = .011, N = 7). (c-d), Flow cytometric assessment of macrophage subpopulations: M1 macrophages (MHC-II/CD86 high) (**p = .002), (c), M2 macrophages (MHC-II/CD86 low) (**p = .0001), (d), ratio M1/M2 macrophages (***p = .0001), (e). Mann–Whitney test was used.