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. 2019 Nov 4;8(1):1574–1583. doi: 10.1080/22221751.2019.1682948

Figure 2.

Figure 2.

Correlation between fluorescence intensity and viral replication capability of CHIKV-iRFP. (A,B) In vitro correlation between fluorescence intensity and viral replication capability. BHK-21 cells in a 12-well plate were infected with CHIKV-iRFP at the 0.1, 0.5, 2.5 MOI, respectively. At 48 hpi, the plate was imaged (A), and the culture media were collected and subjected to plaque assay. Fluorescence radiant efficiency quantified by Living Image software was plotted against viral titre in the culture media (B). (C,D) In vivo correlation between fluorescence intensity and viral replication capability. One-day-old BALB/c mice were infected through i.c. injection with the indicated doses of CHIKV-iRFP. Representative images from three mice were shown at 24 hpi using IVIS Spectrum instrument equipped with 675/30 nm excitation and 720/20 nm emission filters. The colour bar indicates the fluorescence radiant efficiency (C). Total fluorescence radiant efficiency of the head areas of the iRFP expressing mice was plotted against inoculation dose (D).