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. 2019 Nov 4;8(1):1574–1583. doi: 10.1080/22221751.2019.1682948

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Age-dependent replication in suckling BALB/c mice. (A) Time-course whole-body fluorescence images in suckling BALB/c mice at different ages. Suckling BALB/c mice (n = 5 or 6) of indicated ages were infected through i.c. injection with CHIKV-iRFP (10⁴ PFU) diluted in 20 μl of DMEM and were imaged at the indicated time points. (B) Total fluorescence radiant efficiency of the head areas of the iRFP expressing mice in (A) was quantified using Living Image software. (C) Survival curve of the iRFP expressing mice in (A). (D) Time-course body weight changes in suckling mice in (A).