Figure 5.

In vivo imaging of CHIKV-iRFP dynamics and localization. (A) Viral dynamics and localization in infected A129 mice. A129 mice were infected through left footpad with CHIKV-iRFP (10³ PFU) diluted in 50 μl of DMEM and imaged at the indicated time points with head fur removed using a depilatory cream. (B) Fluorescence signals of mice in (A) were quantified using Living Image software. (C) The skeletons were imaged after euthanasia of dying mice. (D) Fluorescence signals of mice in (C) were quantified using Living Image software. (E) Bone marrow cells were harvested from CHIKV-iRFP, CHIKV and mock infected A129 mice after euthanasia and IFA was performed on the bone marrow smears using anti-CHIKV polyclonal antibody. (F) A129 mice were euthanized at 5 dpi and tissues were isolated and homogenized. Plaque assays were performed to quantify viral load in tissues.