Figure 4.

KFX modulated inflammatory factors expression in WIRS-induced gastric ulcer rats. (A and B) The gastric tissue homogenate was prepared to determine the levels of TNF-α, MMP-2, PGE2 and NO by ELISA. (C) The gastric samples were prepared with lysis buffer, resolved on 10% SDS-PAGE, and transferred to a PVDF membrane. Western blots were performed to detect the total or MMP-9, IL-6 and using specific primary antibodies. (D) Densitometric analysis was used to quantify the levels of TNF-α, IL-6 and MMP-9. (E) The total RNA in gastric tissues was extracted to detect the COX-1, COX-2, IL-6, TNF-α, MMP-9, and GAPDH mRNAs by QPCR. The data were expressed as the means ± SD (n = 5). ^##p < 0.01, compared with the control group; *p < 0.05, **p < 0.01 compared with the model group. KFX: Kangfuxin liquid; SJWT: Sanjiuweitai Granules; OME: Omeprazole.