Figure 1.

Serum extracellular vesicles isolated from healthy volunteers are a mixture of exosomes and other microvesicles.

(a) A representative transmission electron micrograph (TEM) of EVs from patient serum is shown (scale bar = 200nm), an inset shows a zoomed in view of particles (scale bar = 100 nm). (b) Dynamic light scattering of serum EVs shows that the average size of isolated particles to be around 200nm. (c) Flow cytometric analyses of beads incubated with EVs shows the detection of Annexin V, CD81 and ApoB with fluorochrome-labelled primary antibodies. Annexin V, CD81 and ApoB are shown in the histograms in blue while the isotype controls are depicted in red. (d) Dot Blot showing the expression of Alix and ApoB in EV’s isolated from Serum using the ExoEasy protocol. Serum was blotted as a positive control for ApoB.