. 2019 Nov 3;10(1):604–634. doi: 10.1080/21655979.2019.1684607

Table 4.

DSMZ nutrient media for the Methanothermobacter genus (141. for Defluvii and thermoflexus) (119. for Crinale, Marburgensis, Tenebrarum, Thermoautotrophicus, Thermophilus, wolfeii).

141. METHANOGENIUM MEDIUM (/L dist. H₂O)		119. METHANOBACTERIUM MEDIUM (/L dist. H₂O)
KCl	3.40 x 10⁻¹ g	KH₂PO₄	5.38 x 10⁻¹ g
MgCl2 x 6 H2O	4.00 x 10° g
MgSO4 x 7 H2O	3.45 x 10° g	MgSO₄ x 7 H₂O	4.30 x 10⁻¹ g
NH4Cl	2.50 x 10⁻¹ g	NH₄Cl	4.30 x 10⁻¹ g
CaCl2 x 2 H2O	1.40 x 10⁻¹ g	CaCl2 x 2 H₂O	5.38 x 10⁻² g
K2HPO4	1.40 x 10⁻¹ g
NaCl	1.80 x 10¹ g	NaCl	4.30 x 10⁻¹ g
Trace element solution	1.00 x 10¹ ml	Trace element solution SL-10	1.08 x 10° ml
Fe(NH4)2(SO4)2 x 6 H2O solution	2.00 x 10° ml
Na-acetate	1.00 x 10° g	Na-acetate	1.08 x 10° g
Yeast extract (OXOID)	2.00 x 10° g	Yeast extract (OXOID)	1.08 x 10° g
Trypticase peptone (BD BBL)	2.00 x 10° g
Na-resazurin solution (0.1% w/v)	5.00 x 10⁻¹ ml	Na-resazurin solution (0.1% w/v)	5.38 x 10⁻¹ ml
NaHCO3	5.00 x 10° g	NaHCO₃	4.30 x 10° g
Vitamin solution	1.00 x 10¹ ml
L-Cysteine-HCl x H2O	5.00 x 10⁻¹ g	L-Cysteine-HCl x H₂O	5.38 x 10⁻¹ g
Na2S x 9 H2O	5.00 x 10⁻¹ g	Na₂S x 9 H₂O	5.38 x 10⁻¹ g
		Fatty acid mixture	2.15 x 10¹ ml
		Na-formate	2.15 x 10° g
		Sludge fluid	5.38 x 10¹ ml
		FeSO₄ x 7 H₂O solution (0.1% w/v in 0.1 N H₂SO4)	2.15 x 10° ml
		KH₂PO₄	5.38 x 10⁻¹ g