. 2019 Nov 2;9(1):1683935. doi: 10.1080/20008686.2019.1683935

Table 2.

Real-time PCR assays used to detect Bartonella spp., Francisella spp. and T. gondii in I. ricinus.

	Sequence (5ʹ-3ʹ)	Target gene	Size of amplicon (bp)	Reference
Bartonella spp.		ssrA¹	301	Diaz et al. [20]
Forward	GCT ATG GTA ATA AAT GGA CAA TGA AAT AA
Reverse	GCT TCT GTT GCC AGG TG
Francisella spp.		sucC²	125	This study
Forward	AAC TGG CTG ACC TTC AGC AT
Reverse	GTG GTC GTG GTA AAG CTG GT
Probe	FAM³-CCG ATT AGG CTT TCT GCT ACT TCA CGA-BHQ1⁴
Forward (PHV⁵)	GGG CGA ATC ACA GAT TGA ATC
Reverse (PHV)	GCG GTT CCA AAC GTA CCA A
Probe (PHV)	TR⁶-TTT TTA TGT GTC CGC CAC CAT CTG GAT C-BHQ1
T. gondii		B1⁷	98	Lin et al. [22]
Forward	TCC CCT CTG GCG AAA AGT
Reverse	AGC GTT CGT GGT CAA CTA TCG ATT G
Probe	FAM-TCT GTG CAA CTT TGG TGT ATT CGC AG-TAMRA⁸