Fig 3. Comparison of lncRNA expression in T_N and T_CM T-cells.

Expression of PVT1, RP11-347C18.3, RP11-539L10.2 and NRON was measured by ddPCR and normalized to expression of the housekeeping gene RPL27. Six independent experiments were performed using uninfected CD4+ T-cells. Significance was determined using a paired two-sided t-test for log₂ transformed data. Data is presented as individual data points (copy numbers normalized to RPL27) symbol-coded by donor, mean of all values is shown. Samples were generated using blood from donors who were different from those who participated in the study that used models of HIV latency. T_N, naïve CD4+ T-cells; T_CM, central memory CD4+ T-cells.