Fig 7. Down-regulation of PVT1, RP11-347C18.3 and NRON by SAHA and RMD.

Mock-infected cells and the bystander model of HIV latency were treated with SAHA (1 μM), RMD (15 nM) or their solvent DMSO for 24 hours. Expression of lncRNAs was measured by ddPCR and normalized to expression of the housekeeping gene RPL27. Six replicate experiments were performed. Significance was determined by implementing repeated measures analysis of variance (RM ANOVA) with library nlme in R using log₂ transformed data. Data is presented as individual data points (copy numbers normalized to RPL27) symbol-coded by donor, mean of all values is shown. DMSO, dimethyl sulfoxide; SAHA, suberoylanilide hydroxamic acid; RMD, Romidepsin; ns, not significant (p-value > 0.05).