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. 2019 Nov 11;14(11):e0224846. doi: 10.1371/journal.pone.0224846

Fig 4. Characterization of H9-derived hNPs by immunocytochemistry at an early stage.

Fig 4

Neuronal progenitors were plated on matrigel-coated ibidi chambers or PDL/Laminin coated-12 mm coverslips on day 23. Cells were fed with NDM every 2 days. At Days 13–28, most H9-derived cells expressed the primary progenitor and telencephalon marker FOXG1 (A) and the neural stem cell markers nestin (B) and PAX6 (C); cells immunoreactive for the stem cell marker Oct4 were rare (data not shown). On Day 41 (E-F), some cells were TUJ1, MAP2 and SMI-312 positive but vGAT, vGLUT1 and synapsin 1 signals were absent (data not shown). Scale bars: A, 25μm; B-D and F, 100μm; E, 50μm.