Figure 3.

GCA regulated imatinib resistance in CML patient-derived primary leukemia cells. (a) PBMCs derived from 5 independent CML-CP patients were transfected with control and GCA siRNAs and (b) PBMCs from 5 independent CML-CP patients were transfected with MYC-tagged GCA (MYC-GCA) expression plasmid. The cells were treated with imatinib (0, 250 nM) for 48 h and stained with ANXA5 for FACS analysis. All graphs illustrated the ratio (%) of ANXA5-positive apoptotic cells. The graphs show the average of three independent experiments. The error bars indicate the S.D. Student’s t-test, **p < 0.01.