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. 2019 Apr 16;15(12):2063–2075. doi: 10.1080/15548627.2019.1596491

Figure 1.

Figure 1.

Number of autophagic cells decreased after induction of KLF2. (a) Shows the MDC staining of RAW264.7 cells at various time points during the osteoclastic differentiation in presence or absence of 3 MA (autophagy inhibitor) or GGTI298 (KLF2 inducer), or RAW264.7 cells without differentiation as a control. (b) MDC stained vesicles were visualized in Klf2 overexpressed (Ad-Klf2) or empty vector (EV) infected control cells; and Klf2 knockdown (siKlf2) or control non-specific siRNA (NS) transfected cells during the course of osteoclast differentiation. (c) Bar graphs represent the cumulative data in percent of autophagic cells present per high power field of image. Each experiment was performed at least 3 times and 15 fields were counted/experiment. (d) Bar graphs represent the quantification of autophagic cells in (Ad-Klf2/EV) infected or (siKlf2/NS) transfected RAW264.7 cells during the course of differentiation. Each experiment was performed at least 3 times and 15 fields were counted/experiment. Data represent as Mean±SEM.