Figure 5.

Arthritis reduces KLF2 and induces expression of osteoclast- and autophagy-related molecules. (a) Images of K/BxN serum-induced arthritic inflammation of limbs in C57/BL6 male mice. (b) Measured ankle thickness during the course of arthritis development shown graphically. Arrowheads indicate the location of measured area (ankle). (c) Expression levels of osteoclast- and autophagy-related molecules from bone marrow monocytes from RA and control mice are shown graphically after correcting with the corresponding internal control β-Actin expressions. (d) Autophagic vesicles are detected with MDC staining in differentiated osteoclasts (Day 6) from the bone marrow precursor cells of mice after induction of RA or without induction of RA as a control.