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. 2019 Mar 31;15(12):2126–2141. doi: 10.1080/15548627.2019.1596496

Figure 4.

Figure 4.

Treatment of ProUBQ10::GFP-ATG8a transgenic Arabidopsis seedlings with anti-microfilament drugs has no effect on salt stress-induced autophagy. (a) Representative confocal images of GFP-ATG8a-labeled autophagic structures in drug-treated root cortex cells. To block autophagic flux, roots were treated with or without 1 μM Con A for 12 h. Roots were treated with 150 mM NaCl plus anti-microfilament drugs for 1 h. Scale bars: 20 μm. (b) Number of autophagic structures per field in each treatment. More than 40 root cortex cells were quantified in each treatment. Values represent means ± SE from 3 independent experiments. Different letters indicate significant difference (ANOVA, p < 0.05). (c) GFP-ATG8 processing assay of seedling in each treatment. Open and closed arrowheads indicate the GFP band and GFP-ATG8a band respectively. Ponceau staining indicates equal loading (lower panel). (d) Quantification of the GFP/GFP-ATG8a ratio shown in (c). Values represent means ± SE from 3 independent experiments. No significant difference was detected among these samples (ANOVA, p > 0.05).